UHPLC Column, RP C4 Butyl, 1.8um, 100A. 2.1mm ID x 150mm Length. Cogent Brand. 1 EA.

Additional Information:

Cogent RP C4 Butyl is a reversed-phase HPLC and UHPLC stationary phase designed for the separation of hydrophobic to moderately hydrophobic compounds. Manufactured using a traditional butyl (C4) bonded phase on ultra-high purity silica and fully double end capped, it provides reproducible retention, excellent peak shape, and reduced secondary interactions. The shorter alkyl chain offers less retention than C8 or C18 phases, making it well suited for fast analyses, biomolecules, peptides, proteins, and method development where excessive retention is undesirable.

68104-15P-2 is a UHPLC column packed with 1.8 µm RP C4 Butyl particles featuring a 100 Å pore size in a 2.1 mm ID x 150 mm format. The 150 mm length maximizes resolution and peak capacity for complex separations, while the 1.8 µm particle technology delivers exceptional efficiency and chromatographic performance. This column is specifically engineered for high-pressure UHPLC applications and is intended to take full advantage of UHPLC instrumentation capable of operating at the elevated pressures generated by sub-2 µm particles. Typical operating flow rates are approximately 0.2–0.4 mL/min. Because of the low flow rates required by 2.1 mm ID columns, any HPLC system used with this column must be capable of delivering accurate and reproducible low-flow performance.

Ideal for very hydrophobic and neutral compounds, this column provides maximum chromatographic efficiency, increased peak capacity, excellent resolution of complex mixtures, reduced solvent consumption, and enhanced laboratory productivity. The 2.1 mm ID format is particularly well suited for LC-MS applications. While compatible HPLC systems may be used for certain methods, this column is designed primarily for UHPLC operation where the full benefits of sub-2 µm particle technology—including superior efficiency, higher resolution, increased sensitivity, and faster separations—can be fully realized.

Key Benefits

  • RP C4 Butyl stationary phase

  • Fully double end capped

  • Traditional butyl bonded phase

  • Ultra-high purity silica support

  • Engineered for UHPLC applications

  • 1.8 µm particle technology

  • Exceptional column efficiency

  • Increased peak capacity

  • Maximum chromatographic resolution

  • Reduced solvent consumption

Typical Applications

  • UHPLC analysis

  • High-pressure liquid chromatography

  • LC-MS methods

  • High-resolution separations

  • Method development

  • Peptide analysis

  • Protein analysis

  • Hydrophobic compound analysis

  • Neutral compound analysis

  • Pharmaceutical analysis

Specifications

  • Product Type: UHPLC Column

  • Stationary Phase: RP C4 Butyl

  • Chromatography Mode: Reversed Phase

  • Bonded Phase: Butyl (C4)

  • Endcapping: Fully Double End Capped

  • Particle Size: 1.8 µm

  • Pore Size: 100 Å

  • Column Dimensions: 2.1 mm ID x 150 mm

  • Length: 150 mm

  • Typical Flow Rate: 0.2–0.4 mL/min

  • Brand: Cogent

Important Notes

  • Specifically designed for UHPLC operation and high-pressure reversed-phase separations

  • Delivers maximum performance when used on a UHPLC system designed for sub-2 µm particle columns

  • May be used on compatible HPLC systems that can reliably operate at the required pressures and low flow rates

  • Sub-2 µm particles generate significantly higher backpressure than conventional HPLC columns

  • The 2.1 mm ID format is ideal for LC-MS applications and reduced solvent consumption

  • Standard HPLC systems must be capable of precise and reproducible operation at approximately 0.2–0.4 mL/min

  • The 150 mm length maximizes resolution and peak capacity for complex separations

  • Samples should be filtered through a 0.2 µm syringe filter prior to injection

  • Lower retention than comparable C8 or C18 stationary phases

  • Ideal when the highest chromatographic efficiency and resolving power are required


 

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